Auxotrophy-Based Detection of Hyperornithinemia in Mouse Blood and Urine

Gyrate atrophy of the choroid and retina (GACR) is a hereditary form of progressive blindness caused by homozygosity for loss-of-function mutations in the ornithine aminotransferase gene ( Oat ).The high levels of circulating ornithine that 7gm pravana lead to ophthalmic symptoms in young adults are also displayed by 2 ornithine aminotransferase (OAT)-deficient mouse models of GACR.Here, we have developed an inexpensive and quantitative bacteria-based test for detecting hyperornithinemia in blood mpu63zm/a or urine samples from these mutant mice, a test that we suggest could be used to facilitate the identification and treatment of OAT-deficient humans before the onset of visual impairment.